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The influence of metal substitution to the catalytic constants for NagA could be utilized to evaluate the contributions created by these divalent cations on enzymatic activity (22, 23). The relationship on the worth of k
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m profile (Table 3). These effects are fully in keeping with the conclusion that the next ionization noticed within the pH-fee profile for log k
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Web-site particular mutants of NagA had been produced so as to verify the roles of precise residues in substrate recognition and catalytic functionality. The metallic material as well as kinetic parameters to the mutants of NagA produced for this investigation are introduced in Table 4.
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